A new disease, bacterial fruit rot of jujube, caused by Xanthomonas arboricola in Korea

Jujube (Zizyphus jujuba) is an economically important crop in Korea. Jujube fruits are used as an ingredient in Korean cooking and as a medicine. A fruit rot of jujube was observed in fields in the Boeun District and in Kyungsan City, Korea in 2008 and 2009. Initial symptoms on fruit included small, circular reddish to black spots which turned deep brown to red brown as they expanded (Figs. 1A, 1B). The surface of affected areas eventually became sunken, while internal tissues turned deep brown (Figs. 1C, 1D). Ten bacterial isolates were obtained from the diseased fruits (surface-sterilised in 70% ethanol for 1 min) by plating on trypticase soy agar. All isolates were Gram-negative aerobic rods with a single flagellum. Colonies were yellow and raised with smooth margins on peptone sucrose agar.

To complete Koch's postulates, detached jujube fruits (cv. Bokjoe) wounded with a razor blade were spot-inoculated with 5 µl of a cell suspension containing 10⁵ cfu/ ml (five fruits per isolate). The fruits were maintained in a plastic box with high humidity at 28ºC. Inoculated surfaces turned brown in 24 hrs followed by internal discoloration within 25 days. Bacteria were reisolated and their identities confirmed by metabolic fingerprinting using the Biolog Microbial Identification System, version 4.2 (Biolog Inc., Hayward, CA, USA), and re-inoculation inoculation of fruits as described above. No symptoms were noted on two fruits inoculated with sterile distilled water (Fig. 2). The gyrB region was partially sequenced to aid in identification of three isolates, BC2927, BC2928 and BC3042, using PCR primers reported by Young et al. (2008). A 860-bp fragment of the gyrB of the three isolates was compared with sequences of the reference strains of the genus Xanthomonas available in the GenBank. The isolates were clustered with X. arboricola pathovars, and clearly separated all the pathovars in a phylogenetic tree generated by the neighbour-joining method in the MEGA software, version 4.1 (Fig. 3) (Tamura et al., 2007).The gyrB sequencefrom the isolates had distance indices of 0.014, 0.017, 0.017, 0.037, 0.013, 0.016, and 0.020, as determined by the Jukes-Cantor model, with sequences of the reference strains of X. arboricola pvs. juglandis, pruni, corylina, populi, celebensis, and the X. arboricola strains from Capsicum spp.(Myung et al., 2010; Young et al., 2008), respectively.On the basis of the sequence, the three isolates were identified as X. arboricola. The disease is named bacterial fruit rot. To our knowledge, this is the first report of bacterial fruit rot of jujube caused by X. arboricola. Further studies are required to determine the pathovar status of the strain. Further spread of the pathogen is expected to have an economic impact on fruit production in Korea. Nucleotide sequence data are available under the following GenBank Accession Nos.: GU120086, GU120087 and GU120085 for gyrB of isolates BC2927, BC2928 and BC3042, respectively.

Figure 1: Symptoms of bacterial fruit rot, caused by Xanthomonas arboricola on jujube: early stage (A), one month later (B), advanced stage (C), internal symptoms (D)

Figure 2: Internal symptoms in detached jujube fruits (cv. Bokjoe), one month after inoculation with 5 µl of a cell suspension (10⁵ cfu/ ml) of Xanthomonas arboricola in sterile distilled water (left), and with sterile distilled water (right)

Figure 3: Phylogenetic tree derived from the neighbour joining model in MEGA software (Version 4.1) based on partial sequences of gyrB of Xanthomonas arboricola strains, BC2927, BC2928 and BC3042 from jujube, X. arboricola strains from bell pepper, and reference strains of the genus Xanthomonas. Number represents bootstrap values based on 1,000 samples.

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References

Myung IS, Jeong IH, Moon SY, Lee SW, Shim HS, 2010. A new disease, arboricola leaf spot of bell pepper, caused by Xanthomonas arboricola. Plant Disease 94, 271. [doi:10.1094/PDIS-94-2-0271C]
Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596-1599. [doi:10.1093/molbev/msm092]
Young JM, Park DC, Shearman HM, Fargier E, 2008. A multilocus sequence analysis of the genus Xanthomonas. Systematic and Applied Microbiology 31, 366-377. [doi:10.1016/j.syapm.2008.06.004]

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To cite this report:

Myung I-, Moon SY, Jeong IH, Lee SW, Ra D-, 2010. A new disease, bacterial fruit rot of jujube, caused by Xanthomonas arboricola in Korea. New Disease Reports 22, 12. [doi:10.5197/j.2044-0588.2010.022.012]

©2010 The Authors